Active substances: Ciprofloxacin
To enforce correct antibiotic use and all antibiotic treatments should be registered of antibiotics, there is a need central database. Metrics details Abstract In The Netherlands, registration, and to enforce prudent use at the farm and in a for methods that are able to.
The mobile phase consists of 2 mM ammonium formate and 0.
The gradient: 0—1. The gradient is returned to its initial conditions within 0.
The flow rate is 0. The operating parameters are: capillary voltage. The antibiotics were fragmented using collision induced dissociation argon.
SRM transitions were selected based on the abundance of the signal and, if multiple options were available, the selectivity of the transition Table 1. Data were acquired and processed using MassLynx 4. The full validation was carried out using the worst-case scenario: determination of total residue as described above as this will result in the most challenging sample extracts excluding washing and including grinding.
Limit of detection LOD and limit of confirmation LOC were additionally determined for the analysis of freely extractable residues. In order to critically assess the applicability of the method, quantitative confirmatory parameters were assessed additionally.
Ultimately, a validation is carried out using incurred certified reference materials. Because these are not available for this specific application, the second best option was used: spiking.
Since there is no MRL or other target level for antibiotics in feathers, target levels were chosen based on MRLs in other animal matrices and the performance of the method during method development.
The validation was carried out at three different levels 0. Quantitative results were obtained through correction with the corresponding isotopically labeled internal standards, if available. In case no isotopically labeled internal standard was available, corrections were made using another internal standard.