Active substances: Doxycycline
In addition to their antibiotic activity, TCs and their chemically modified analogues CMTs are known to suppress bone resorption, both in vitro and in vivo. At first, the inhibition of bone loss was solely attributed to the ability of TCs to inhibit the enzymatic action of matrix metalloproteinases MMPs, especially collagenases and gelatinases.
However, Bettany et al. Furthermore, following the latter study, Holmes et al. This accumulating evidence suggested that TCs or CMTs can suppress bone resorption by inhibiting not only the bone lytic enzyme activity of osteoclasts i.
Animals were housed and maintained in 12-hour:12-hour light-dark cycle laboratory lighting, unless otherwise noted. Magnetic resonance imaging examination occurred 6 to 8 weeks after cessation of doxycycline treatment.
Magnetic Resonance Imaging The general mouse preparation for high resolution MRI is well established in our laboratory. In other words, to compensate for reduced signal to noise ratios at shorter TRs, progressively more images were collected as the TR decreased.
During an MRI session, animals were studied in an alternating order between controls and experimental mice. They have proven valuable for studying the roles of activated G-protein signaling in complex systems, including cardiomyocyte function, neurological development and function, and bone development.
Since many GPCRs show both constitutive and ligand-activated signaling, having temporal and tissue-specific control of GPCR transgene expression is important for delineating specific signaling functions.
Various methods are available for introducing the responder and regulator constructs independently for example, by mixing lentiviral constructs or sequential transgene introduction.
To address these challenges and determine if global Gs signaling affects early mouse development, we developed a single-vector polycistronic Tet-inducible expression platform based on a modular construction strategy.
This platform uses three vectors that can be recombined to form a single Tet-inducible expression vector.
In this study, we demonstrate the utility of our single-vector constructs in mouse embryonic stem ES cells and mice.
Finally, our results indicate that low-level expression of a GPCR with constitutive Gs activity leads to a mild increase in calvarial bone formation. The construct was verified by sequencing. The construct was fully sequence verified.
The self-cleaving 2 A site generates two separate peptides in equal concentrations via a ribosomal "skip" mechanism just before the C-terminal end of the 2 A peptide and has been useful for making multicistronic reporters.